Journal
/
발생학
/
Upright Imaging of Drosophila Egg Chambers
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Upright Imaging of Drosophila Egg Chambers

Upright Imaging of Drosophila Egg Chambers

DOI:
10.3791/52636-v

•

12:29 min

•

March 13, 2015

•

,
1Department of Biological Sciences,University of Maryland Baltimore County

Chapters

  • 00:05Title
  • 01:47Ovariole Dissection
  • 03:25Ovariole Immunofluorescent Staining
  • 05:36Glycerin Jelly Slide Preparation
  • 06:57Egg Chamber Mounting
  • 09:22Egg Chamber Imaging Preparation
  • 10:35Results: Enhanced Imaging with the Upright Technique
  • 11:56Conclusion

Summary

자동 번역

자동 번역

The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

Tags

Keywords: DrosophilaEgg ChambersOogenesisFollicle CellsUpright ImagingConfocal MicroscopyGlycerin JellyMounting TechniqueDevelopmental ProcessesCell DifferentiationCell OrganizationMolecular MarkersEgg Development

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/52636?article_type=v

Live-imaging of the Drosophila Pupal Eye
check_url/kr/52636?article_type=v

Imaging Calcium in Drosophila at Egg Activation
check_url/kr/52636?article_type=v

Long-term Live Imaging of Drosophila Eye Disc
check_url/kr/52636?article_type=v

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
check_url/kr/52636?article_type=v

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
check_url/kr/52636?article_type=v

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo
check_url/kr/52636?article_type=v

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines
check_url/kr/52636?article_type=v

Human Egg Maturity Assessment and Its Clinical Application
check_url/kr/52636?article_type=v

Imaging Dpp Release from a Drosophila Wing Disc
check_url/kr/52636?article_type=v

Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

Read Article