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Upright Imaging of Drosophila Egg Chambers
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发育生物学
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JoVE 杂志 发育生物学
Upright Imaging of Drosophila Egg Chambers

Upright Imaging of Drosophila Egg Chambers

DOI:
10.3791/52636-v

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12:29 min

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March 13, 2015

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,
1Department of Biological Sciences,University of Maryland Baltimore County

Chapters

  • 00:05Title
  • 01:47Ovariole Dissection
  • 03:25Ovariole Immunofluorescent Staining
  • 05:36Glycerin Jelly Slide Preparation
  • 06:57Egg Chamber Mounting
  • 09:22Egg Chamber Imaging Preparation
  • 10:35Results: Enhanced Imaging with the Upright Technique
  • 11:56Conclusion

Summary

自动翻译

自动翻译

The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.

Tags

Keywords: DrosophilaEgg ChambersOogenesisFollicle CellsUpright ImagingConfocal MicroscopyGlycerin JellyMounting TechniqueDevelopmental ProcessesCell DifferentiationCell OrganizationMolecular MarkersEgg Development

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