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Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
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1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales,Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química,Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular,Cinvestav-IPN, 6Subd. de Investigación,Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud
Chapters
- 00:05Title
- 01:15Isolation of Human Mesenchymal Stem Cells
- 03:20Expansion of Mesenchymal Stem Cells
- 04:37Preparation of Porous Bone Matrix Disk and Culture of Human Mesenchymal Stem Cells
- 05:54Cell Proliferation Assay and Measuring Colony Forming Units
- 06:56Results: Characterization of Isolated Human Mesenchymal Stem Cells
- 08:30Conclusion
Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.
