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Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
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発生生物学
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JoVE Journal 発生生物学
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

27,171 Views

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09:00 min

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February 09, 2015

DOI:

10.3791/51999-v

DOI:
10.3791/51999-v

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09:00 min
February 09, 2015

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15 Views
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1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales,Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química,Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular,Cinvestav-IPN, 6Subd. de Investigación,Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

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Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.

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Mesenchymal Stem CellsAM-hMSCsBone MatrixNukboneScaffoldCell DifferentiationBiomaterialsCell Culture

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Rodríguez-Fuentes, N., Reynoso-Ducoing, O., Rodríguez-Hernández, A., Ambrosio-Hernández, J. R., Piña-Barba, M. C., Zepeda-Rodríguez, A., Cerbón-Cervantes, M. A., Tapia-Ramírez, J., Alcantara-Quintana, L. E. Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix. J. Vis. Exp. (96), e51999, doi:10.3791/51999 (2015).

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