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Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
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Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

14,642 Views

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12:19 min

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November 19, 2014

DOI:

10.3791/51972-v

DOI:
10.3791/51972-v

•

12:19 min
November 19, 2014

•

22 Views
, , ,
1Department of Physiology,University of California Los Angeles, 2Department of Neurobiology,University of California Los Angeles

Summary

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The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.

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Intracellular CalciumAstrocytesStriatumGenetically-encoded Calcium IndicatorsGCaMPIn VivoIn SituConfocal ImagingMicrocircuitsCalcium Signaling

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Jiang, R., Haustein, M. D., Sofroniew, M. V., Khakh, B. S. Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators. J. Vis. Exp. (93), e51972, doi:10.3791/51972 (2014).

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