ジャーナル
/
神経科学
/
Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

14,642 Views

•

12:19 min

•

November 19, 2014

DOI:

10.3791/51972-v

DOI:
10.3791/51972-v

•

12:19 min
November 19, 2014

•

22 Views
, , ,
1生理学科,University of California Los Angeles, 2Department of Neurobiology,University of California Los Angeles

概要

Automatically generated

The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.

Explore More Videos

Intracellular CalciumAstrocytesStriatumGenetically-encoded Calcium IndicatorsGCaMPIn VivoIn SituConfocal ImagingMicrocircuitsCalcium Signaling

Read Article

Cite this Article

Jiang, R., Haustein, M. D., Sofroniew, M. V., Khakh, B. S. Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators. J. Vis. Exp. (93), e51972, doi:10.3791/51972 (2014).

Download .ris file

Copy

Share Video

.

Copy