An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.
Huiskonen, J. T., Parsy, M., Li, S., Bitto, D., Renner, M., Bowden, T. A. Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo. J. Vis. Exp. (92), e51714, doi:10.3791/51714 (2014).