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Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

11,891 Views

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08:29 min

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October 21, 2014

DOI:

10.3791/51714-v

DOI:
10.3791/51714-v

•

08:29 min
October 21, 2014

•

2 Views
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1Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford

Summary

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An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.

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Keyword Extraction:Viral Envelope Glycoprotein SpikesElectron CryotomographyJsubtomoViral PathogenesisDrug DesignElectron Cryo-tomographyTomogramsSub-tomogramsBunyamwera VirusBunyaviridae

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Huiskonen, J. T., Parsy, M., Li, S., Bitto, D., Renner, M., Bowden, T. A. Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo. J. Vis. Exp. (92), e51714, doi:10.3791/51714 (2014).

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