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Immunologie und Infektion
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Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

DOI:
10.3791/51714-v

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08:29 min

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October 21, 2014

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, , , , ,
1Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford

Kapitel

  • 00:05Titel
  • 01:46Extracting Virus Sub‐volumes from Full‐size Tomograms
  • 02:32Generation of Two Independent Initial Models
  • 03:58Gold‐standard Iterative Alignment and Averaging of the Two Initial Spike Models & Generation and Alignment of Seeds to the Virus Surface for Template Matching
  • 05:48Gold‐standard Iterative Alignment and Averaging of the Spike Structure and Visualization of the Results
  • 06:44Results: Structure of the Envelope Glycoprotein complex of Bunyamwera Virus
  • 07:54Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package Jsubtomo.

Tags

Keyword Extraction:Viral Envelope Glycoprotein SpikesElectron CryotomographyJsubtomoViral PathogenesisDrug DesignElectron Cryo-tomographyTomogramsSub-tomogramsBunyamwera VirusBunyaviridae

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