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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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July 11, 2014
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The reliability of results in metabolomics experiments depends on the effectiveness and reproducibility of the sample preparation. Described is a rigorous and in-depth method that enables extraction of metabolites from biological fluids with the option of subsequently analyzing up to thousands of compounds, or just the compound classes of interest.
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Cruickshank-Quinn, C., Quinn, K. D., Powell, R., Yang, Y., Armstrong, M., Mahaffey, S., Reisdorph, R., Reisdorph, N. Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis. J. Vis. Exp. (89), e51670, doi:10.3791/51670 (2014).
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