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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

JoVE Journal
Bioengenharia

This content is Free Access.

JoVE Journal Bioengenharia

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

31,382 Views

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11:25 min

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July 11, 2014

DOI:

10.3791/51670-v

DOI:

10.3791/51670-v

•

11:25 min

July 11, 2014

•

4 Views

Charmion Cruickshank-Quinn, Kevin D. Quinn, Roger Powell, Yanhui Yang, Michael Armstrong, Spencer Mahaffey, Richard Reisdorph, Nichole Reisdorph

¹Department of Immunology,National Jewish Health, ²Department of Pharmacology, School of Medicine,University of Colorado Denver

Tags

Metabolomics Sample Preparation Protein Precipitation Liquid-liquid Extraction Solid-phase Extraction Metabolite Fractionation Hydrophilic Hydrophobic Fatty Acids Neutral Lipids Phospholipids Plasma Bronchoalveolar Lavage Fluid Cerebrospinal Fluid

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