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High-throughput Analysis of Mammalian Olfactory Receptors
 

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

Article DOI: 10.3791/51640-v 12:02 min June 2nd, 2014
June 2nd, 2014

챕터

요약

Olfactory receptor activation patterns encode odor identity, but the lack of published data identifying odorant ligands for mammalian olfactory receptors hinders the development of a comprehensive model of odor coding. This protocol describes a method to facilitate high-throughput identification of olfactory receptor ligands and quantification of receptor activation.

Tags

High-throughput Analysis Mammalian Olfactory Receptors Receptor Activation Luciferase Activity Odorants Olfactory Receptor Family Hana3A Cells Luciferase Reporter Assay Dose Response Curves Receptor Variants Screen Odorant/receptor Pairs Response Scores Odorant Coding
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