/
/
High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
Kapitel
- 00:05Titel
- 01:28Plating Cells for Transfection
- 02:57Transfection of Olfactory Receptors
- 04:52Odor Stimulation
- 05:52Measuring Olfactory Receptor Activity via Luciferase Assay
- 09:50Results: Characterization of Odorant/Olfactory Receptor Interactions
- 11:27Conclusion
Olfactory receptor activation patterns encode odor identity, but the lack of published data identifying odorant ligands for mammalian olfactory receptors hinders the development of a comprehensive model of odor coding. This protocol describes a method to facilitate high-throughput identification of olfactory receptor ligands and quantification of receptor activation.
