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Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
 

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Article DOI: 10.3791/51576-v • 14:45 min • August 6th, 2014
August 6th, 2014 •
1, 1, 1, 1, 1, 1, 1
1Environmental Health Science and Research Bureau, Health Canada, Environmental Health Centre

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요약
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De novo mutations in the male germline may contribute to adverse health outcomes in subsequent generations. Here we describe a protocol for the use of a transgenic rodent model for quantifying mutations in male germ cells induced by environmental agents.

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Transgenic Rodent Assay Male Germ Cell Mutant Frequency De Novo Mutations Adverse Health Outcomes Traditional Methods Large Numbers Of Animals Impractical Germ Cell Mutagenicity Chemical Testing Risk Assessment In Vivo Male Germ Cell Mutation Assay Transgenic Rodent Model Organisation For Economic Co-operation And Development (OECD) Test Guideline In Vitro Positive Selection Assay Reporter Gene Germ Cells Of Exposed Males Stage-specific Sensitivity Spermatogenic Cell Types Mutagen Exposure Experimental Parameters Duration Of Exposure Administration Time Time Between Exposure And Sample Collection Sampling Time Cell Population Collected For Analysis Superior Sensitivity Time And Resources
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