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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
Chapters
- 00:05Title
- 01:45Spinal Cord and Brain Tissue Preparation
- 03:28Myelin Flotation
- 04:33Sucrose Gradient Fractionation
- 06:23RNA Isolation from Individual Fractions
- 07:36RNA Profiles from Spinal Cord and Brain Tissue
- 08:25Conclusion
This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.
