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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
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April 30, 2014
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This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.
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Lou, W. P., Baser, A., Klußmann, S., Martin-Villalba, A. In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation. J. Vis. Exp. (86), e51255, doi:10.3791/51255 (2014).
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