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JoVE Journal Neuroscience
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
 

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Article DOI: 10.3791/51255-v • 09:13 min • April 30th, 2014
April 30th, 2014 •
1, 1, 1, 1
1Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)

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This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.

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In Vivo Central Nervous System Translatome Polyribosome Fractionation Gene Expression Transcription Translation MRNA Stability Protein Abundance Regulatory Mechanisms MRNA Translation Translatomics Neurobiology Polyribosome Fractionation Technique Actively Translated MRNAs Ribosomes Sucrose Gradients In Vivo Samples Central Nervous System (CNS) Fatty Tissue Components CNS Material Mouse Spinal Cord Brain Cycloheximide Myelin Flotation
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