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Anti-Nuclear Antibody Screening Using HEp-2 Cells
Chapters
- 00:05Title
- 02:11Sample Preparation and Substrate Selection
- 03:08Addition of Controls and Samples
- 04:34Addition of Fluorescent Conjugate
- 06:08Identification of Positive and Negative Results
- 07:52Results: Cell Staining Patterns for HEp-2
- 11:23Conclusion
Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.
