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Anti-Nuclear Antibody Screening Using HEp-2 Cells
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Journal JoVE Biologie
Anti-Nuclear Antibody Screening Using HEp-2 Cells

Anti-Nuclear Antibody Screening Using HEp-2 Cells

132,411 Views

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13:01 min

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June 23, 2014

DOI:

10.3791/51211-v

DOI:
10.3791/51211-v

•

13:01 min
June 23, 2014

•

39 Views
, , ,
1IFA Development Manager,INOVA Diagnostics, Inc., 2IFA Development,INOVA Diagnostics, Inc., 3Product Manager, Rheumatology,INOVA Diagnostics, Inc., 4Medical Director,INOVA Diagnostics, Inc.

Summary

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Indirect immunofluorescent (IIF) assays have traditionally been used for the detection of antinuclear antibodies (ANA) in human serum. The presence of these antibodies can aid in the diagnosis of systemic autoimmune rheumatic diseases (SARD). This protocol demonstrates how to effectively perform the IIF technique to accurately detect these autoantibodies.

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Cite this Article

Buchner, C., Bryant, C., Eslami, A., Lakos, G. Anti-Nuclear Antibody Screening Using HEp-2 Cells. J. Vis. Exp. (88), e51211, doi:10.3791/51211 (2014).

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