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Identificatie van post-translationele modificaties van Plant eiwitcomplexen
JoVE 신문
생물학
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JoVE 신문 생물학
Identification of Post-translational Modifications of Plant Protein Complexes
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Identificatie van post-translationele modificaties van Plant eiwitcomplexen

23,475 Views

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10:07 min

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February 22, 2014

DOI:

10.3791/51095-v

DOI:
10.3791/51095-v

•

10:07 min
February 22, 2014

•

8 Views
, , , , ,
1School of Life Sciences,University of Warwick, 2The Sainsbury Laboratory,Norwich Research Park, 3Research School of Biology,The Australian National University

Summary

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We beschrijven hier een protocol voor de zuivering en karakterisering van plantaardig eiwit complexen. We zien dat door immunoprecipitatie een eiwit in een complex, zodat we kunnen zijn post-translationele modificaties en zijn interactie partners identificeren.

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Plant ProteinsPost-translational ModificationsPTMsProtein ComplexesImmune ComplexesNB-LRR ProteinsMass SpectrometryProtein PurificationImmunoprecipitation

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Cite this Article

Piquerez, S. J. M., Balmuth, A. L., Sklenář, J., Jones, A. M., Rathjen, J. P., Ntoukakis, V. Identification of Post-translational Modifications of Plant Protein Complexes. J. Vis. Exp. (84), e51095, doi:10.3791/51095 (2014).

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