Rivista
/
Biologia
/
Identification of Post-translational Modifications of Plant Protein Complexes
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

DOI:
10.3791/51095-v

•

10:07 min

•

February 22, 2014

•

, , , , ,
1School of Life Sciences,University of Warwick, 2The Sainsbury Laboratory,Norwich Research Park, 3Research School of Biology,The Australian National University

Capitoli

  • 00:05Titolo
  • 01:37N. benthamiana and A. thaliana Protein Samples
  • 03:24Protein Immunoprecipitation
  • 05:32Protein Digestion
  • 07:48Results: Identification of Post-translational Modifications of Tagged Proteins
  • 09:13Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.

Tags

Plant ProteinsPost-translational ModificationsPTMsProtein ComplexesImmune ComplexesNB-LRR ProteinsMass SpectrometryProtein PurificationImmunoprecipitation

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Detection of Histone Modifications in Plant Leaves

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Read Article