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Identification of Post-translational Modifications of Plant Protein Complexes
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Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

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10:07 min

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February 22, 2014

DOI:

10.3791/51095-v

DOI:
10.3791/51095-v

•

10:07 min
February 22, 2014

•

8 Views
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1School of Life Sciences,University of Warwick, 2The Sainsbury Laboratory,Norwich Research Park, 3Research School of Biology,The Australian National University

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We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.

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Plant ProteinsPost-translational ModificationsPTMsProtein ComplexesImmune ComplexesNB-LRR ProteinsMass SpectrometryProtein PurificationImmunoprecipitation

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Piquerez, S. J. M., Balmuth, A. L., Sklenář, J., Jones, A. M., Rathjen, J. P., Ntoukakis, V. Identification of Post-translational Modifications of Plant Protein Complexes. J. Vis. Exp. (84), e51095, doi:10.3791/51095 (2014).

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