Journal
/
신경과학
/
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

13,026 Views

•

10:51 min

•

November 25, 2013

DOI:

10.3791/50905-v

DOI:
10.3791/50905-v

•

10:51 min
November 25, 2013

•

2 Views
, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

Summary

Automatically generated

Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

Explore More Videos

Subventricular ZoneNeurogenic NicheNeuroblast MigrationRostral Migratory StreamOlfactory BulbIn Vivo ElectroporationTime-lapse ImagingAcute Brain SlicesNeurogenesisBrain Repair

Read Article

Cite this Article

Sonego, M., Zhou, Y., Oudin, M. J., Doherty, P., Lalli, G. In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices. J. Vis. Exp. (81), e50905, doi:10.3791/50905 (2013).

Download .ris file

Copy

Share Video

.

Copy