ジャーナル
/
神経科学
/
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

13,026 Views

•

10:51 min

•

November 25, 2013

DOI:

10.3791/50905-v

DOI:
10.3791/50905-v

•

10:51 min
November 25, 2013

•

2 Views
, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

概要

Automatically generated

Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

Explore More Videos

Subventricular ZoneNeurogenic NicheNeuroblast MigrationRostral Migratory StreamOlfactory BulbIn Vivo ElectroporationTime-lapse ImagingAcute Brain SlicesNeurogenesisBrain Repair

Read Article

Cite this Article

Sonego, M., Zhou, Y., Oudin, M. J., Doherty, P., Lalli, G. In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices. J. Vis. Exp. (81), e50905, doi:10.3791/50905 (2013).

Download .ris file

Copy

Share Video

.

Copy