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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

13,595 Views

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11:10 min

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November 27, 2013

DOI:

10.3791/50861-v

DOI:
10.3791/50861-v

•

11:10 min
November 27, 2013

•

6 Views
, ,
1Department of Pharmacology and Physiology,Rutgers New Jersey Medical School, 2Center for Taste and Feeding Behavior,Universite de Bourgogne

Summary

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The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.

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Membrane Potential DyeVentromedial HypothalamusGlucose SensingAdult MiceFluorescence ImagingNeuronal ActivityElectrophysiologyHeterogeneous Neuronal PopulationEnergy BalanceObesity

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Cite this Article

Vazirani, R. P., Fioramonti, X., Routh, V. H. Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing. J. Vis. Exp. (81), e50861, doi:10.3791/50861 (2013).

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