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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
Chapters
- 00:05Title
- 01:18Brain Slicing and Dissection
- 02:35Dissociation and Culture
- 05:50Preparation for Membrane Potential Dye Imaging
- 08:23MPD Imaging
- 09:36Results: Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons
- 10:43Conclusion
The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.
