Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Reema P. Vazirani; Xavier Fioramonti; Vanessa H. Routh

doi:10.3791/50861

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神経科学

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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

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神経科学

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JoVE Journal 神経科学

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

13,595 Views

•

11:10 min

•

November 27, 2013

DOI:

10.3791/50861-v

DOI:

10.3791/50861-v

•

11:10 min

November 27, 2013

•

6 Views

Reema P. Vazirani¹, Xavier Fioramonti², Vanessa H. Routh¹

¹Department of Pharmacology and Physiology,Rutgers New Jersey Medical School, ²Center for Taste and Feeding Behavior,Universite de Bourgogne

概要

The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.

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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

概要

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