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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

43,053 Views

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07:23 min

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September 05, 2013

DOI:

10.3791/50729-v

DOI:
10.3791/50729-v

•

07:23 min
September 05, 2013

•

20 Views
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1Department of Microbiology, Immunology, and Cancer Biology,University of Virginia Health Sciences Center

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Fluorescence MicroscopyBacterial ViabilityMammalian CellsNeisseria GonorrhoeaeSYTO9DAPIPropidium IodideSYTOX GreenGentamicin Protection AssayElectron MicroscopyBacterial PathogenesisBacterial LocalizationHost-pathogen Interactions

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Johnson, M. B., Criss, A. K. Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells. J. Vis. Exp. (79), e50729, doi:10.3791/50729 (2013).

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