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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
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JoVE Revista Inmunología y contagio
Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

43,028 Views

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07:23 min

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September 05, 2013

DOI:

10.3791/50729-v

DOI:
10.3791/50729-v

•

07:23 min
September 05, 2013

•

20 Views
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1Department of Microbiology, Immunology, and Cancer Biology,University of Virginia Health Sciences Center

Tags

Fluorescence MicroscopyBacterial ViabilityMammalian CellsNeisseria GonorrhoeaeSYTO9DAPIPropidium IodideSYTOX GreenGentamicin Protection AssayElectron MicroscopyBacterial PathogenesisBacterial LocalizationHost-pathogen Interactions

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Johnson, M. B., Criss, A. K. Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells. J. Vis. Exp. (79), e50729, doi:10.3791/50729 (2013).

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