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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
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September 05, 2013
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Central to the field of bacterial pathogenesis is the ability to define if and how microbes survive after exposure to eukaryotic cells. This article outlines protocols for the use of fluorescent dyes that reveal the viability of individual bacteria inside and associated with host cells.
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Johnson, M. B., Criss, A. K. Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells. J. Vis. Exp. (79), e50729, doi:10.3791/50729 (2013).
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