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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
Chapters
- 00:05Title
- 01:37Assessing Bacterial Viability: Propidium Iodide and SYTO9
- 02:56Assessing Bacterial Viability: Subcellular Localization
- 04:52Results: Human Neutrophils and Survival of N. gonorrhoeae
- 06:52Conclusion
Central to the field of bacterial pathogenesis is the ability to define if and how microbes survive after exposure to eukaryotic cells. This article outlines protocols for the use of fluorescent dyes that reveal the viability of individual bacteria inside and associated with host cells.
