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Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:02Zebrafish Care and Maintenance
- 01:59Microplate Reader Programming
- 03:57Fluorescence Quantification
- 04:34Data Analysis
- 05:41Results: Respiratory Burst Response in Zebrafish Embryos
- 06:41Conclusion
The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.
