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Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
Chapters
- 00:05Title
- 01:02Zebrafish Care and Maintenance
- 01:59Microplate Reader Programming
- 03:57Fluorescence Quantification
- 04:34Data Analysis
- 05:41Results: Respiratory Burst Response in Zebrafish Embryos
- 06:41Conclusion
The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.
