The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.
Goody, M. F., Peterman, E., Sullivan, C., Kim, C. H. Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish. J. Vis. Exp. (79), e50667, doi:10.3791/50667 (2013).