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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Chapters
- 00:05Title
- 01:40Isolation of Mouse Bone Marrow Cells
- 04:14Neutrophil Density Gradient Centrifugation Separation and CellTracker Dye-labeling
- 05:50Results: Bone Marrow-isolated Neutrophils are Highly Purified, Highly Viable, and Trackable by Dye-labeling in Various Mouse Tissues after Adoptive Transfer
- 07:07Conclusion
We describe a protocol for isolation and purification of neutrophils from mouse bone marrow by density gradient centrifugation and for neutrophil labeling using CellTracker dyes. This represents a simple, fast, reproducible and economical method for obtaining large numbers of neutrophils for downstream functional studies or adoptive transfer and tracking experiments.
