04:14Neutrophil Density Gradient Centrifugation Separation and CellTracker Dye-labeling
05:50Results: Bone Marrow-isolated Neutrophils are Highly Purified, Highly Viable, and Trackable by Dye-labeling in Various Mouse Tissues after Adoptive Transfer
We describe a protocol for isolation and purification of neutrophils from mouse bone marrow by density gradient centrifugation and for neutrophil labeling using CellTracker dyes. This represents a simple, fast, reproducible and economical method for obtaining large numbers of neutrophils for downstream functional studies or adoptive transfer and tracking experiments.