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遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔
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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔

DOI:
10.3791/50412-v

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11:48 min

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July 24, 2013

•

, , ,
1Institute of Anatomy and Cell Biology,University of Heidelberg , 2Institut de recherches cliniques de Montreal

Chapters

  • 00:05Title
  • 02:06Preparation of DNA and Glass Micropipettes for Injection
  • 02:57Laparotomy
  • 04:33DNA Injection into the Brain Ventricle
  • 05:59Targeting the Hypothalamus for Electroporation
  • 07:17Finishing the Surgery
  • 08:28Analyzing the Results
  • 09:18Results: Hypothalamus Transfection by In utero Electroporation
  • 10:54Conclusion

Summary

자동 번역

자동 번역

尽管下丘脑的功能和医学重要性，<em>在子宫内</em>遗传操纵其发展已经很少有人尝试。我们显示的详细步骤<em>在子宫内</em>电到老鼠的下丘脑和展示具有代表性的结果整体和局部（区域）下丘脑转。

Tags

Genetic ManipulationMouseDeveloping HypothalamusIn Utero ElectroporationGenetic ModificationMammalian BrainMouse MutantsDeveloping BrainCortexThalamusHypothalamusHypothalamic NeuroepitheliumMammillary BodyElectroporation

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