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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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神经科学
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JoVE 杂志 神经科学
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

15,666 Views

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11:48 min

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July 24, 2013

DOI:

10.3791/50412-v

DOI:
10.3791/50412-v

•

11:48 min
July 24, 2013

•

1 Views
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1Institute of Anatomy and Cell Biology,University of Heidelberg , 2Institut de recherches cliniques de Montreal

Summary

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Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

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Cite this Article

Haddad-Tóvolli, R., Szabó, N., Zhou, X., Alvarez-Bolado, G. Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation. J. Vis. Exp. (77), e50412, doi:10.3791/50412 (2013).

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