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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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July 24, 2013
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Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.
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Haddad-Tóvolli, R., Szabó, N., Zhou, X., Alvarez-Bolado, G. Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation. J. Vis. Exp. (77), e50412, doi:10.3791/50412 (2013).
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