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In Vivo Modeling of the Morbid Human Genome using Danio rerio
 

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Article DOI: 10.3791/50338-v • 12:31 min • August 24th, 2013
August 24th, 2013 •
1,2, 1,3, 1, 1, 1, 1
1Center for Human Disease Modeling, Department of Cell Biology, Duke University Medical Center, 2Department of Evolutionary Anthropology, Duke University, 3Department of Pediatrics, Duke University Medical Center

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Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.

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In Vivo Modeling Morbid Human Genome Danio Rerio Zebrafish Assays Clinically Significant Nonsynonymous Changes In Vivo Complementation Experimental Tractability Embryogenesis Molecular Processes Morphogenetic Signaling Developmental Defects In Pediatric Disease Adult-onset Disorders Zebrafish Genome Conservation Recapitulate Human Disease States Dominant Negative Alleles Gain Of Function Alleles Morpholino Antisense Oligonucleotides Loss Of Function Variants Human Protein Sequence Rescue Phenotype
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