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An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
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An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
Chapters
- 00:05Title
- 01:09PBMC Isolation (Histopaque) and Cell Counting
- 02:55Negative Isolation of Monocytes
- 05:09Results: Representative Primary Human Macrophages
- 07:15Conclusion
Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.
