/
/
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
18,460 Views
•
07:42 min
•
June 12, 2013
DOI:
Özet
Automatically generated
Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.
Read Article
Cite this Article
Erbel, C., Rupp, G., Helmes, C. M., Tyka, M., Linden, F., Doesch, A. O., Katus, H. A., Gleissner, C. A. An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization. J. Vis. Exp. (76), e50332, doi:10.3791/50332 (2013).
Copy