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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

DOI:
10.3791/50321-v

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10:49 min

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April 14, 2013

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, , ,
1Department of Neuroscience,The University of Connecticut Health Center, 2Department of Genetics and Developmental Biology,The University of Connecticut Health Center, 3Stem Cell Institute,The University of Connecticut Health Center

Chapters

  • 00:05Title
  • 01:39Generation of Human Pluripotent Stem Cell Aggregates
  • 03:53Induction of Neuroepithelial Cells
  • 05:11Generation of Telencephalic Progenitors
  • 06:33Differentiation into Telencephalic Neurons
  • 08:18Telencephalic Neuronal Generation
  • 10:11Conclusion

Summary

자동 번역

자동 번역

This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

Tags

Telencephalic Glutamatergic NeuronsHuman Pluripotent Stem CellsDifferentiation ProcessClumpsAggregatesSuspension CultureHESC MediumSpontaneous DifferentiationThree Germ LayersNeural Induction MediumNeuroepithelial CellsNeurospheresCoverslipsTelencephalic PrecursorsDorsal Telencephalic ProgenitorsGlutamatergic NeuronsDevelopment Of NeuronsDiseases Affecting Neurons

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