Journal
/
생물학
/
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

19,955 Views

•

10:53 min

•

July 03, 2013

DOI:

10.3791/50235-v

DOI:
10.3791/50235-v

•

10:53 min
July 03, 2013

•

4 Views
, ,
1Institute of Pharmacology,University of Duisburg-Essen , 2Division of Experimental Cardiology,University of Heidelberg

Summary

Automatically generated

We describe the isolation of human atrial myocytes which can be used for intracellular Ca2+ measurements in combination with electrophysiological patch-clamp studies.

Explore More Videos

Human Atrial MyocytesPatch-clampCa2+ TransientsMembrane CurrentsAtrial FibrillationCell IsolationCollagenaseProteaseCa2+ Buffering

Read Article

Cite this Article

Voigt, N., Zhou, X., Dobrev, D. Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. J. Vis. Exp. (77), e50235, doi:10.3791/50235 (2013).

Download .ris file

Copy

Share Video

.

Copy