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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

19,955 Views

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10:53 min

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July 03, 2013

DOI:

10.3791/50235-v

DOI:
10.3791/50235-v

•

10:53 min
July 03, 2013

•

4 Views
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1Institute of Pharmacology,University of Duisburg-Essen , 2Division of Experimental Cardiology,University of Heidelberg

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We describe the isolation of human atrial myocytes which can be used for intracellular Ca2+ measurements in combination with electrophysiological patch-clamp studies.

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Human Atrial MyocytesPatch-clampCa2+ TransientsMembrane CurrentsAtrial FibrillationCell IsolationCollagenaseProteaseCa2+ Buffering

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Voigt, N., Zhou, X., Dobrev, D. Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. J. Vis. Exp. (77), e50235, doi:10.3791/50235 (2013).

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