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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

20,197 Views

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16:07 min

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March 30, 2013

DOI:

10.3791/50180-v

DOI:
10.3791/50180-v

•

16:07 min
March 30, 2013

•

9 Views
, , ,
1Center for Human Genetic Research and Department of Medicine,Massachusetts General Hospital and Harvard Medical School, 2Department of Earth, Atmospheric, and Planetary Sciences,Massachusetts Institute of Technology

Summary

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We present robust biochemical and microscopic methods for studying Caenorhabditis elegans lipid stores. A rapid, simple, fixing-staining procedure for fluorescent lipid droplet imaging leverages the spectral properties of the lipophilic dye Nile red. We then present biochemical measurement of triglycerides and phospholipids using solid phase extraction and gas chromatography-mass spectrometry.

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Caenorhabditis ElegansLipid DropletsNile Red StainingHigh-throughput MicroscopyTriglyceridesPhospholipidsGas Chromatography-mass Spectrometry

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Pino, E. C., Webster, C. M., Carr, C. E., Soukas, A. A. Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans. J. Vis. Exp. (73), e50180, doi:10.3791/50180 (2013).

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