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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

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16:07 min

March 30, 2013

DOI:

16:07 min
March 30, 2013

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概要

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We present robust biochemical and microscopic methods for studying Caenorhabditis elegans lipid stores. A rapid, simple, fixing-staining procedure for fluorescent lipid droplet imaging leverages the spectral properties of the lipophilic dye Nile red. We then present biochemical measurement of triglycerides and phospholipids using solid phase extraction and gas chromatography-mass spectrometry.

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