ジャーナル
/
生物学
/
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

20,168 Views

•

16:07 min

•

March 30, 2013

DOI:

10.3791/50180-v

DOI:
10.3791/50180-v

•

16:07 min
March 30, 2013

•

9 Views
, , ,
1Center for Human Genetic Research and Department of Medicine,Massachusetts General Hospital and Harvard Medical School, 2Department of Earth, Atmospheric, and Planetary Sciences,Massachusetts Institute of Technology

概要

Automatically generated

We present robust biochemical and microscopic methods for studying Caenorhabditis elegans lipid stores. A rapid, simple, fixing-staining procedure for fluorescent lipid droplet imaging leverages the spectral properties of the lipophilic dye Nile red. We then present biochemical measurement of triglycerides and phospholipids using solid phase extraction and gas chromatography-mass spectrometry.

Explore More Videos

Caenorhabditis ElegansLipid DropletsNile Red StainingHigh-throughput MicroscopyTriglyceridesPhospholipidsGas Chromatography-mass Spectrometry

Read Article

Cite this Article

Pino, E. C., Webster, C. M., Carr, C. E., Soukas, A. A. Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans. J. Vis. Exp. (73), e50180, doi:10.3791/50180 (2013).

Download .ris file

Copy

Share Video

.

Copy