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的视觉检测，以监测T6SS介导的细菌竞争
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Visual Assay to Monitor T6SS-mediated Bacterial Competition
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的视觉检测，以监测T6SS介导的细菌竞争

15,045 Views

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08:45 min

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March 20, 2013

DOI:

10.3791/50103-v

DOI:
10.3791/50103-v

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08:45 min
March 20, 2013

•

9 Views
, ,
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology,Imperial College London

Summary

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我们描述了定性检测，监测细菌介导的竞争<em>铜绿假单胞菌</em>类型的VI分泌系统（T6SS）的。分析依赖的生存/杀死大肠杆菌靶细胞背着<em> lacZ的</em>记者。这种技术是可调的，以评估杀菌/抑菌活性精通T6SS的微生物。

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Cite this Article

Hachani, A., Lossi, N. S., Filloux, A. A Visual Assay to Monitor T6SS-mediated Bacterial Competition. J. Vis. Exp. (73), e50103, doi:10.3791/50103 (2013).

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