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A Visual Assay to Monitor T6SS-mediated Bacterial Competition
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March 20, 2013
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We describe a qualitative assay to monitor bacterial competition mediated by the Pseudomonas aeruginosa type VI secretion system (T6SS). The assay relies on the survival/killing of Escherichia coli target cells carrying a lacZ-reporter. This technique is adjustable to assess the bactericidal/bacteriostasis activity of T6SS-proficient microorganisms.
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Hachani, A., Lossi, N. S., Filloux, A. A Visual Assay to Monitor T6SS-mediated Bacterial Competition. J. Vis. Exp. (73), e50103, doi:10.3791/50103 (2013).
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