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A Visual Assay to Monitor T6SS-mediated Bacterial Competition
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
A Visual Assay to Monitor T6SS-mediated Bacterial Competition

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

15,129 Views

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08:45 min

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March 20, 2013

DOI:

10.3791/50103-v

DOI:
10.3791/50103-v

•

08:45 min
March 20, 2013

•

9 Views
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1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology,Imperial College London

Summary

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We describe a qualitative assay to monitor bacterial competition mediated by the Pseudomonas aeruginosa type VI secretion system (T6SS). The assay relies on the survival/killing of Escherichia coli target cells carrying a lacZ-reporter. This technique is adjustable to assess the bactericidal/bacteriostasis activity of T6SS-proficient microorganisms.

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Cite this Article

Hachani, A., Lossi, N. S., Filloux, A. A Visual Assay to Monitor T6SS-mediated Bacterial Competition. J. Vis. Exp. (73), e50103, doi:10.3791/50103 (2013).

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