Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

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Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

DOI:

10.3791/4254-v

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12:44 min

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October 11, 2012

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Jennifer L. Fogel, Thu Zan Tun Thein, Francesca V. Mariani

¹Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Chapters

00:05Title
01:48LysoTracker Staining of Mouse Embryos
04:53LysoTracker Staining of Differentiating ES Cultures
06:51Mounting Samples for Imaging
07:44Viewing LysoTracker-stained Samples and Image Analysis
09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
12:10Conclusion

Summary

자동 번역

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

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Chapters

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