Rivista
/
Biologia
/
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

DOI:
10.3791/4254-v

•

12:44 min

•

October 11, 2012

•

, ,
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Capitoli

  • 00:05Titolo
  • 01:48LysoTracker Staining of Mouse Embryos
  • 04:53LysoTracker Staining of Differentiating ES Cultures
  • 06:51Mounting Samples for Imaging
  • 07:44Viewing LysoTracker-stained Samples and Image Analysis
  • 09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
  • 12:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

Tags

LysoTrackerProgrammed Cell DeathPCDEmbryosDifferentiating Embryonic Stem CellsApoptosisCaspase Enzyme CascadeMembrane BlebbingNuclear ShrinkingDNA FragmentationAutophagyImmune CellsNon-professional PhagocytesLysosomal Activity

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/4254?article_type=v

Dissection of 6.5 dpc Mouse Embryos
check_url/it/4254?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/it/4254?article_type=v

Derivation of Human Embryonic Stem Cells by Immunosurgery
check_url/it/4254?article_type=v

Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia
check_url/it/4254?article_type=v

Freezing and Thawing Human Embryonic Stem Cells
check_url/it/4254?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/it/4254?article_type=v

Isolation and Derivation of Mouse Embryonic Germinal Cells
check_url/it/4254?article_type=v

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
check_url/it/4254?article_type=v

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
check_url/it/4254?article_type=v

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Read Article